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The authors and publisher of this book have a great pleasure and satisfied to present this book, ZOO - 271 - MJP: Practicals in Medical Laboratory Techniques II (P) Semester...

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Practicals in Medical Laboratory Techniques - II
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The authors and publisher of this book have a great pleasure and satisfied to present this book, ZOO - 271 - MJP: Practicals in Medical Laboratory Techniques II (P) Semester IV for S.Y.B.Sc. Zoology students. The book has been written as per the new revised syllabus B.Sc. Zoology Certificate, diploma, Major (03 years) / B.Sc. Honours in Zoology (04 years) for the Colleges Affiliated to Savitribai Phule Pune University, Pune to be implemented from June 2025.
The main aim in writing this book is to provide basic information of the subject to cater the needs of the subject as per National Education Policy (NEP), 2020. We tried to explain the concepts and information in a simple lucid language so that students could understand it easily.

1. To study microscopic examination of acute and chronic inflammation. (D)..............7

2. Preparation of stains used in cytology - eosin, methylene blue, haematoxylin, giemsa (E) (Compulsory)..............................22

3. Prepare permanent slide of tissues by using microtomy. (E) (Compulsory).............31

4. Microscopic examination of stained tissue slides (E)....41

5. To perform bleeding & clotting time from different blood samples. (E) (Compulsory)..................................45

6. To study the different types of cytological specimens used in cytopathology. (D)..................................49

7. Estimation of total proteins by Biuret method and estimation of serum albumin
and serum globulin. (E) (Compulsory)................68

8. To perform assays for liver enzymes (e.g. ALT, AST) and bilirubin........................75

9. Measurement of cholesterol and triglyceride levels. (E).........................81

10. To culture and identify bacteria using various media and biochemical tests. (E).....90

11. To study the types of cancer & common techniques used in cancer cytology. (D).....................................101

12. To study the steps in autopsy. (D).....................104

13. To study the types and steps of biopsy. (D).......106

• ANNEXURE: Methods of cholesterol estimation............110

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