Practical Botany - Based on BOT-251 (M) AND BOT 252 (M)

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This practical manual is designed to introduce students to the laboratory aspects of plant anatomy, embryology, and plant biotechnology. It includes practical exercises related to the study of plant tissues...

Name : Practical Botany - Based on BOT-251 (M) AND BOT 252 (M)
Vendor : Prashant Publication
Type : S.Y.B.Sc. | Sem. IV | BOT-253 (MJP)
Barcode : 9788199852624

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INTERNATIONAL	XS	S	M	L	XL	XXL	XXXL
EUROPE	32	34	36	38	40	42	44
US	0	2	4	6	8	10	12
CHEST FIT (INCHES)	28"	30"	32"	34"	36"	38"	40"
CHEST FIT (CM)	716	76	81	86	91.5	96.5	101.1
WAIST FIR (INCHES)	21"	23"	25"	27"	29"	31"	33"
WAIST FIR (CM)	53.5	58.5	63.5	68.5	74	79	84
HIPS FIR (INCHES)	33"	34"	36"	38"	40"	42"	44"
HIPS FIR (CM)	81.5	86.5	91.5	96.5	101	106.5	111.5
SKORT LENGTHS (SM)	36.5	38	39.5	41	42.5	44	45.5

This practical manual is designed to introduce students to the laboratory aspects of plant anatomy, embryology, and plant biotechnology. It includes practical exercises related to the study of plant tissues and tissue systems, secondary growth, reproductive structures, embryological development, as well as basic techniques and applications of plant biotechnology such as tissue culture, SEP technology, Enzyme and Fermentation Technology related protocols. Each practical has been presented in a systematic manner with aims, requirements, procedures, observations, and suitable diagrams wherever necessary, so as to make learning more effective and student-friendly.
Special care has been taken to align the contents strictly with the prescribed syllabus of Savitribai Phule Pune University. The inclusion of clear illustrations and simple explanations will help students to understand complex anatomical and developmental concepts with ease and will also assist teachers during practical demonstrations.

•Introduction.......................................................07
Credit I – Practical Based on BOT 251 MJ (Plant Anatomy and Embryology)
1. Study of epidermal tissue system-Epidermis, stomata and epidermal outgrowths.......12

2. Study of the distribution of mechanical tissues in root, stem and leaf; and principles involved in it..........16

3. Study of normal secondary growth in dicot stem – Annona and Helianthus. (Double stained temporary preparation).............20

4. Study of anomalous secondary growth in Bignonia and Dracaena stem. (Double stained temporary preparation)....................26

5. Demonstration of T.S. of tetrasporangiate anther and types of ovules.........................30

6. Study of in vivo pollen grain germination in any suitable plant...................................35

7. Demonstration of types of endosperms and embryos. .......................37
Credit II– Practical Based on BOT 252 MJ (Plant Biotechnology)

8. Instrument / equipment used in plant tissue culture – principle and working of autoclave, hot air oven, laminar air flow, pH meter...................43

9. Production of SCP using Spirulina / Yeast and study of its commercial
product (Demonstration)..................................49

10. Preparation of MS medium and inoculation of nodal sector / apical meristem/embryo............................................55

11. Production of citric acid by Aspergillus niger and estimation of citric acid
by titration method............................................66

12. To extract the enzyme catalase from a suitable plant material and to study its enzymatic activity.......71

13. To immobilize the enzyme amylase (or yeast invertase) using sodium alginate
and calcium chloride and to demonstrate the activity of the immobilized enzyme...........73

14. Visit to tissue culture / biotechnology industry / fermentation industry / effluent treatment plant and submission of visit report............................75