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This book have a great pleasure and satisfied to present this book, ZOO – 151 – CELL BIOLOGY AND BIOMEDICAL TECHNIQUES (T) for F.Y.B.Sc. (SEM II) Zoology students as one...

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Cell Biology And Biomedical Techniques
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This book have a great pleasure and satisfied to present this book, ZOO – 151 – CELL BIOLOGY AND BIOMEDICAL TECHNIQUES (T) for F.Y.B.Sc. (SEM II) Zoology students as one of the major subject. The book has been written as per the new revised syllabus B. Sc. Zoology Certificate, diploma, Major (03 years) / B.Sc. Honours in Zoology (04 years) for the Colleges Affiliated to Savitribai Phule Pune University, Pune to be implemented from June 2024. The main aim in writing this book is to provide basic information of the subject to cater the needs of the subject as per National Education Policy (NEP), 2020. We tried to explain the concepts and information in a simple lucid language so that students could understand it easily. The better understanding of students. The figures and photographs included in each chapter will help the students to grasp the subject matter. The authors are extremely thankful to Dr. R. D. Chaudhari, Chairman and also to other members in B.O.S. Zoology for their needy help and support in publishing this book.We are thankful to the authors of the book and web link pages mentioned in the reference section of this book which we referred in particular chapter.

1. Chapter………………………………………………. 7
Overview of Cells
1.1 Prokaryotic (E. coli) and Eukaryotic (Plant & Animal) cells.
1.2 Microscopy – Simple and compound microscope.
1.3 Micrometry.
1.4 Types of Stains: Acidic, basic and neutral.

2. Chapter…………………………………….. 33
Plasma Membrane
2.1 Models of plasma membrane.
2.2 Transport across membranes: Active and passive transport, facilitated transport, endocytosis, exocytosis.
2.3 Cell – Cell Junction, Structure and function, tight junctions, adherent junctions, gap junctions, desmosomes and hemi-desmosomes.

3. Chapter……………………………………….. 48
Cell Organelles: Structure and Functions
3.1 Nucleus and nuclear pore complex.
3.2 Endoplasmic reticulum.
3.2 Golgi complex.
3.3 Lysosomes.
3.4 Ribosome.
3.5 Peroxisomes.
3.6 Mitochondria.

4. Chapter………………………………………………….. 79
Cell Cycle, Cell Division : Mitosis and Meiosis
4.1 Cell cycle.
4.2 Mitosis.
4.3 Meiosis.

5. Chapter…………………………………………………………….. 95
Introduction and Scope of Biomedical Techniques
5.1 Lab Safety Techniques
5.2 Sterilization Techniques

6. Chapter………………………………………… 120
Laboratory Instruments
Introduction, principle and working
6.1 Centrifugation.
6.2 Chromatography.
6.3 Spectroscopy.
6.4 Electrophoresis.
6.5 Microtomy.
7. Chapter……………………………………………………………………………………… 142
Biomedical Instruments
Introduction, principle & brief working of
7.1 Electrocardiography (ECG).
7.2 Ultrasound / Sonography.
7.3 Polymerase chain reaction (PCR).

8. Chapter……………………………………………… 153
Clinical Techniques
Introduction and working principle
8.1 Blood collection.
8.2 Anticoagulants.
8.3 Preparation and staining of blood smears.
8.4 Differential leucocyte count.
8.5 Hemocytometry (RBC and WBC).
8.6 Hemoglobin estimation.

9. Chapter………………………………………………………… 185
Urine Analysis
9.1 Collection of urine sample.
9.2 Preservation of urine sample.
9.3 Routine urine analysis – Physical, bio-chemical and microscopic examination.

Annexure……………………………………… 204
Sample Questions With Answers

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