Practicals in Cell Biology and Biomedicaltechniques

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The authors and publisher of this book have a great pleasure and satisfied to present this book, ZOO – 152 – PRACTICALS IN CELL BIOLOGY AND BIOMEDICAL TECHNIQUES (P) for...

Name : Practicals in Cell Biology and Biomedicaltechniques
Vendor : Prashant Publication
Type : F.Y.B.Sc. Sem. II ZOO-152 (P)
Barcode : 9789348537164

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INTERNATIONAL	XS	S	M	L	XL	XXL	XXXL
EUROPE	32	34	36	38	40	42	44
US	0	2	4	6	8	10	12
CHEST FIT (INCHES)	28"	30"	32"	34"	36"	38"	40"
CHEST FIT (CM)	716	76	81	86	91.5	96.5	101.1
WAIST FIR (INCHES)	21"	23"	25"	27"	29"	31"	33"
WAIST FIR (CM)	53.5	58.5	63.5	68.5	74	79	84
HIPS FIR (INCHES)	33"	34"	36"	38"	40"	42"	44"
HIPS FIR (CM)	81.5	86.5	91.5	96.5	101	106.5	111.5
SKORT LENGTHS (SM)	36.5	38	39.5	41	42.5	44	45.5

The authors and publisher of this book have a great pleasure and satisfied to present this book, ZOO – 152 – PRACTICALS IN CELL BIOLOGY AND BIOMEDICAL TECHNIQUES (P) for F.Y.B.Sc. (SEM II) Zoology students as one of the major subject. The book has been written as per the new revised syllabus B.Sc. Zoology Certificate, diploma, Major (03 years) / B. Sc. Honours in Zoology (04 years) for the Colleges Affiliated to Savitribai Phule Pune University, Pune to be implemented from June 2024.

The main aim in writing this book is to provide basic information of the subject to cater the needs of the subject as per National Education Policy (NEP), 2020. We tried to explain the concepts and information in a simple lucid language so that students could understand it easily.

The authors are extremely thankful to Dr. R. D. Chaudhari, Chairman and also to other members in B.O.S. Zoology for their needy help and support in publishing this book.

Practical No. 1…………………………………………………….7
Visualization of animal cells. (E) (Compulsory)

Practical No. 2……………………………………………. 13
Visualization of plant cells. (E) (Compulsory)

Practical No. 3……………………………………………. 18
Study of principle and working of simple and compound microscope. (D)

Practical No. 4…………………………………………………. 24
Measurement of microscopic objects using micrometry. (E) (Compulsory)

Practical No. 5…………………………………………………………. 30
Identification of ultrastructure of different cell organelles from electron micrographs / video. (D)

Practical No. 6…………………………………………………. 41
Staining and visualization of mitochondria by Janus green stain. (E) (Compulsory).

Practical No. 7………………………………………………. 44
Study of permanent slides of mitosis and meiosis. (D).

Practical No. 8………………………………………………. 51
Preparation of temporary stained squash of onion root tip to study various stages of mitosis. (E) (Compulsory)

Practical No. 9………………………………………………. 56
Preparation of temporary stained squash of onion root tip to study various stages of meiosis. (E)

Practical No. 10…………………………………………….. 64
Visualization of nuclear fraction by acetocarmine stain. (E)

Practical No. 11…………………………………………….. 68
Lab safety techniques and sterilization. (D)

Practical No. 12………………………………………….. 79
Study of principle and working of centrifugation and chromatography. (D)

Practical No. 13…………………………………….. 89
Study of principle and working of spectroscopy, electrophoresis and microtomy. (D)

Practical No. 14………………………………………………….. 97
Study of the principle of ECG, ultrasound / sonography, PCR. (D)

Practical No. 15……………………………………………… 110
Hemoglobin estimation using Sahli’s haemometer. (E) (Compulsory)

Practical No. 16………………………………………………… 115
Differential Leucocyte Count. (E) (Compulsory)

Practical No. 17…………………………………………… 123
Total RBC count in blood by using hemocytometer. (E)

Practical No. 18…………………………………………… 130
Total WBC count in blood by using hemocytometer. (E)

Practical No. 19……………………………………… 136
Urine analysis – Normal and abnormal constituents. (E) (Compulsory)

Practical No. 20…………………………… 148
Uric acid crystal examination in urine. (E) (Compulsory)

ANNEXURE…………………………………………………………. 151
A) Preparation of Stains/Solutions/Reagents
B) Sample Questions For Oral
C) Units
D) Suggested Readings and References