Bioinstrumentation
F.Y.B.Sc. Sem. I BT-101 (SEC)
Authors:
ISBN:
Rs.125.00
- DESCRIPTION
- INDEX
We are delighted to present this valuable book on “Bioinstrumentation” toF.Y.B.Sc. Biotechnology students with enormous excitement. This book was put together using Savitribai Phule Pune University’s New Revised Syllabus in keeping with the National Education Policy-2020 (NEP-2020) as of June 2024. Students in the F.Y.B.SC. Biotechnology course (Semester I-2P, under Skill Enhancement Course (SEC)).The purpose of this practical book is to offer teachers the framework they need to organize hands-on activities, effectively involve students in the teaching and learning process, and foster student’s various instrument handling skills. We believe that the book will assist student’s learn different laboratory techniques- Chromatography, Autoclave etc. and gain a brief understanding of the numerous devices -Spectrophotometer, Microscope, etc. and their uses in the laboratory. The most notable aspect of this book is that it offers a thorough strategy in an easy-to-memorize format.The Prashant Publications, Jalgaon, has shown a great deal of interest in publishing this book promptly and in an appealing format and for that we authors feel very grateful. We are thankful for the reader’s precise constructive criticism and suggestions on how to make this book better.
Chapter 1……………………………………………………………………………………………………7
Understanding the laboratory signs and safety:Familiarize students with
common laboratory safety symbols and their meanings, writing log book
Chapter 2………………………………………………………………………………………………….11
Study of component, working and use of Incubator, Distillation Unit,
Laminar air flow, Water bath, Vortex mixer and other miscellaneous
instruments
Chapter 3………………………………………………………………………………………………….23
Study the working and calibration of Electronic Balance
Chapter 4………………………………………………………………………………………………….26
Handling and calibration of Micropipettes
Chapter 5………………………………………………………………………………………………….29
Study the principle and working of pH meter, Preparation of Buffers
Chapter 6………………………………………………………………………………………………….34
Study the working and components of various types of centrifuges
Chapter 7………………………………………………………………………………………………….39
Study of construction and working of Bright field Compound Microscope,
simple Microscope. Observation of basic cell structures using prepared slides
Chapter 8………………………………………………………………………………………………….44
Study the construction and working of Dark field / Phase contrast / Fluorescence microscope. (Demonstration)
Chapter 9………………………………………………………………………………………………….54
Study the principle, construction and working of autoclave and hot air
oven. Sterilization of glassware and reagents using moist heat
Chapter 10………………………………………………………………………………………………..59
Components and working of Colorimetry and preparation of Standard
GraphChapter 11………………………………………………………………………………………………..65
Study the Principle, components and working of Spectrophotometer
Chapter 12………………………………………………………………………………………………..75
Determination of absorption spectra and Lambda max
Chapter 13………………………………………………………………………………………………..79
Separation and identification of amino acids/ sugar by paper chromatography/TLC
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