Practicals in Cell Biology and Biomedicaltechniques – P
F.Y.B.Sc. Sem. II ZOO-152 (P)
Authors:
ISBN:
Rs.225.00
- DESCRIPTION
- INDEX
The authors and publisher of this book have a great pleasure and satisfied
to present this book, ZOO – 152 – PRACTICALS IN CELL BIOLOGY AND
BIOMEDICAL TECHNIQUES (P) for F.Y.B.Sc. (SEM II) Zoology students as
one of the major subject. The book has been written as per the new revised syllabus
B.Sc. Zoology Certificate, diploma, Major (03 years) / B. Sc. Honours in Zoology
(04 years) for the Colleges Affiliated to Savitribai Phule Pune University, Pune to
be implemented from June 2024.
The main aim in writing this book is to provide basic information of the
subject to cater the needs of the subject as per National Education Policy (NEP),
2020. We tried to explain the concepts and information in a simple lucid language
so that students could understand it easily.
We feel that it’s not a new creation but presentation in simple form for the
better understanding of students. The figures and photographs included in each
practical will help the students to grasp the subject matter and will be easy to
perform the practical work.
The authors are extremely thankful to Dr. R. D. Chaudhari, Chairman and
also to other members in B.O.S. Zoology for their needy help and support in
publishing this book.
We are thankful to the authors of the book and web link pages mentioned in
the reference section of this book which we referred in particular chapter.
The authors are also thankful to Mr. Rangrao Patil and the staff of Prashant
Publications, Jalgaon for taking keen interest in publishing this book.
Valuable and constructive suggestions from the teachers and the students
are solicited.
Practical No. 1…………………………………………………………………………………………………….7
Visualization of animal cells. (E) (Compulsory)
Practical No. 2…………………………………………………………………………………………………. 13
Visualization of plant cells. (E) (Compulsory)
Practical No. 3…………………………………………………………………………………………………. 18
Study of principle and working of simple and compound microscope. (D)
Practical No. 4…………………………………………………………………………………………………. 24
Measurement of microscopic objects using micrometry. (E) (Compulsory)
Practical No. 5…………………………………………………………………………………………………. 30
Identification of ultrastructure of different cell organelles from electron micrographs / video. (D)
Practical No. 6…………………………………………………………………………………………………. 41
Staining and visualization of mitochondria by Janus green stain. (E) (Compulsory).
Practical No. 7…………………………………………………………………………………………………. 44
Study of permanent slides of mitosis and meiosis. (D).
Practical No. 8…………………………………………………………………………………………………. 51
Preparation of temporary stained squash of onion root tip to study various stages of mitosis. (E) (Compulsory)
Practical No. 9…………………………………………………………………………………………………. 56
Preparation of temporary stained squash of onion root tip to study various stages of meiosis. (E)
Practical No. 10……………………………………………………………………………………………….. 64
Visualization of nuclear fraction by acetocarmine stain. (E)
Practical No. 11……………………………………………………………………………………………….. 68
Lab safety techniques and sterilization. (D)
Practical No. 12……………………………………………………………………………………………….. 79
Study of principle and working of centrifugation and chromatography. (D)
Practical No. 13……………………………………………………………………………………………….. 89
Study of principle and working of spectroscopy, electrophoresis and microtomy. (D)
Practical No. 14……………………………………………………………………………………………….. 97
Study of the principle of ECG, ultrasound / sonography, PCR. (D)
Practical No. 15……………………………………………………………………………………………… 110
Hemoglobin estimation using Sahli’s haemometer. (E) (Compulsory)
Practical No. 16……………………………………………………………………………………………… 115
Differential Leucocyte Count. (E) (Compulsory)
Practical No. 17……………………………………………………………………………………………… 123
Total RBC count in blood by using hemocytometer. (E)
Practical No. 18……………………………………………………………………………………………… 130
Total WBC count in blood by using hemocytometer. (E)
Practical No. 19……………………………………………………………………………………………… 136
Urine analysis – Normal and abnormal constituents. (E) (Compulsory)
Practical No. 20……………………………………………………………………………………………… 148
Uric acid crystal examination in urine. (E) (Compulsory)
ANNEXURE…………………………………………………………………………………………………. 151
A) Preparation of Stains/Solutions/Reagents
B) Sample Questions For Oral
C) Units
D) Suggested Readings and References
Author
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