1. Introduction to Biotechnology.............................7
1.1 Introduction
1.2 Definition
1.3 Scope of Biotechnology
1.4 Importance of Biotechnology
1.5 Questions
2. Plant Tissue Culture Technology.......................16
2.1 Introduction and Brief History
2.2 Definition and concepts
2.3 Sterilization – Definition, methods
2.4 Nutrient medium – Definition and concept, M.S. medium
2.5 Micropropagation – Definition, concept and stages
2.6 Applications of plant tissue culture
2.7 Questions
3. Single Cell Proteins..........................................39
3.1 Introduction, Concept and Definition
3.2 Importance of SCP in Diet.
3.3 Production of SCP from Spirulina
3.4 Production of SCP from Yeast.
3.5 Economic importance of SCP.
3.6 Acceptability of SCP
3.7 Questions
4. Enzyme Technology..........................................55
4.1 Introduction
4.2 Definition
4.3 Properties of Enzymes
4.4 Classification of Enzymes
4.5 Industrial Applications of Enzymes.
4.6 Production of amylase, proteases and lipase enzyme
Contents
4.7 Enzyme immobilization
4.8 Questions
5. Genetic Engineering Technology.........................79
5.1 Introduction, definition, and concept of genetic engineering.
5.2 Tools of genetic engineering – a) Restriction enzymes and DNA ligases. b) Gene cloning vectors
5.3 Methods of gene transfer in plants – a) Vector mediated gene transfer b) Direct gene transfer- Electroporation, microinjection and Biolistic gene gun.
5.4 Application of plant genetic engineering for crop.
5.5 Questions
6. Fermentation Technology..............................96
6.1 Introduction, Definition and Concept
6.2 Organisms and Substrates of Fermentation
6.3 Types of culture –Batch and Continuous Types of culture
6.4 Liquid and Solid State Fermentation
6.5 Bioreactors in Fermentation
6.6 Concept of Scaling-Up
6.7 Downstream Processing
6.8 Questions
7. Biofuel Technology...................................117
7.1 Introduction: Definition and Concept
7.2 Renewable Energy Sources
7.3 Non Renewable Energy Sources
7.4 Biogas
7.5 Biodiesel
7.6 Biohydrogen
7.7 Questions
