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“Practicals of Microtechnique and Genetics ” ZO- 317 for T.Y.B.Sc. students and teachers of Kavayitri Bahinabai Chaudhari, North Maharashtra University, Jalgaon. It includes Practical based on ZO-314 & 315 as...

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Practicals based on Microtechnique and Genetics - ZO-317
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“Practicals of Microtechnique and Genetics ” ZO- 317 for T.Y.B.Sc. students and teachers of Kavayitri Bahinabai Chaudhari, North Maharashtra University, Jalgaon. It includes Practical based on ZO-314 & 315 as per NEP 2020, w.e.f– June 2026-27. This practical manual furnishes its level best, for the practical information required for completion of practical syllabus of T.Y.B.Sc. Zoology. The students of T.Y.B.Sc. will be definately benefited for their practical knowledge. Each practical is provided with neat, easy and well illustrated labelled diagrams.

Practical – 1...........................................................................7
Study of microtomes- rocking and rotary microtome

Practical – 2........................................................................10
Preparation of compound fixative–Bouin’s fluid and Carnoy’s fluid

Practical – 3.........................................................................12
Study of block preparation and trimming

Practical – 4........................................................................15
Study of section cutting and spreading

Practical – 5........................................................................16
Preparation of slides by double staining method and submission of 5 slides and 5 whole mount

Practical – 6......................................................................20
Vital staining of mitochondria by Janus green B stain

Practical – 7.......................................................................22
Calibration of micrometer Scale of cell diameter from the given permanent slide

Practical – 8......................................................................26
Sketching by camera lucida and measurement of cell diameter from the sketches

Practical – 9......................................................................28
Study of Monohybrid and Dihybrid ratio with suitable example

Practical – 10......................................................................30
Study of Gene Interactions (Non-Mendelian Inheritance) Using Suitable Examples

Practical – 11.....................................................................36
Demonstration and preparation of polytene chromosomes from the salivary gland of Drosophila/Chironomus larvae

Practical – 12.....................................................................39
Study of ABO blood group system in human (ABO and Rh)

Practical – 13.....................................................................42
Study of Human Genetic Traits – Hypertrichosis, PTC Tasting Ability, Widow’s Peak, Tongue Rolling, and Earlobe Attachment

Practical – 14....................................................................46
Determination of Barr body in human buccal epithelial cells

Practical – 15....................................................................48
Analysis of Human Karyotype Spreads for Chromosomal Abnormalities (Klinefelter, Down, Turner, and Patau Syndromes)

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