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This book have a great pleasure and satisfied to present this book, ZOO-306-MJP : Practicals in Animal Physiology & Agrochemicals & Pest Management Semester V for T.Y.B.Sc. Zoology students as...

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Practicals in Animal Physiology & Agrochemicals & Pest Management - ZOO – 306 – MJP
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This book have a great pleasure and satisfied to present this book, ZOO-306-MJP : Practicals in Animal Physiology & Agrochemicals & Pest Management Semester V for T.Y.B.Sc. Zoology students as one of the major subject. The book has been written as per the new revised syllabus B.Sc. Zoology Certificate, diploma, Major (03 years) / B.Sc. Honours in Zoology (04 years) for the Colleges Affiliated to Savitribai Phule Pune University, Pune to be implemented from June 2026.
The main aim in writing this book is to provide basic information of the subject to cater the needs of the subject as per National Education Policy (NEP), 2020. We tried to explain the concepts and information in a simple lucid language so that students could understand it easily.

Practicals in Animal Physiology
1. Estimation of blood group (ABO and Rh factor) using prepared antisera............7

2. Determination of hemoglobin concentration by Sahli’s Hemoglobinometer method. ..........................10

3. Differential leukocyte count (DLC) using prepared blood smear. .....................13

4. Measurement of human physiological parameters: Pulse rate, blood pressure and respiratory rate under resting conditions...................................................16

5. Demonstration of Electrocardiogram (ECG) and interpretation of normal ECG waves - P, QRS and T...........20

6. Qualitative Tests for Food Components: Detection of carbohydrates, proteins and lipids in biological samples...................................23

7. Study of mammalian kidney through charts/models & interpretation of nephron structure & urine formation........................................27

8. Study of skeletal, cardiac and smooth muscles using permanent slides / photomicrographs...............................35

9. Study of nervous tissue, neuron types and neuroglia using permanent slides / charts / models..........40

10. Mini project / Case study report on lifestyle disorders related to physiology - Hypertension, Diabetes, Obesity, Anemia, Kidney Disorders or Cardiovascular Diseases................................................................46

Practicals in Agrochemicals & Pest Management
1. Study of common agrochemicals: Fertilizers, insecticides, fungicides, herbicides and rodenticides using specimens / labels......................................54

2. Identification and classification of common agricultural insect pests from preserved specimens / photographs / charts..................................................58

3. Identification of crop damage symptoms caused by chewing, sucking, boring and leaf-mining pests..............61

4. Preparation of pest surveillance chart for a selected crop using field / photographic observations....................66

5. Calculation of pest density, percentage infestation and preparation of a simple pest monitoring report................69

6. Study of economic threshold level and economic injury level using simple numerical problems............................72

7. Study of pesticide formulations: Dust, granules, wettable powder, emulsifiable concentrate and spray
formulation ......................................75

8. Preparation of working pesticide solution from a given commercial concentration using dilution method.......................................78

9. Study of pesticide label information: Active ingredient, toxicity symbol, dosage, precautions and waiting period.......................................81

10. Study of safety measures and personal protective equipment used during pesticide handling and application...............................................83

11. To study the safe pesticide storage, handling and disposal methods...................85

12. To study the light trap, pheromone trap and yellow sticky trap used in pest monitoring....................................87

13. Study of biological control agents: Predators, parasitoids and pathogens using photographs / specimens / charts...90

14. Preparation of an integrated pest management plan for a selected crop pest......92

15. Detection of pesticides residues in food stuffs...................................94

16. Compulsory field visit to Agricultural field / Nursery / Farm / Krishi Vigyan Kendra / Pesticide Retail Centre to study agrochemicals and pest management
practices ...............................................96

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