The fundamental concept of totipotency that a single cell has the potential to grow into a whole mature plant- is at the heart of plant tissue culture. “ Practice patience and avoid rushing …take a dreep breath, slow down, and remember that patience is your ally.” This speaks to the meticulous, detail oriented work involved in tissue culture, where sterility and precision are critical. S.Y.B.Sc. BOT-242-MNT and BOT-245 MNP Tissue Culture Technology one of such course will make the students understanding about potential of plant cells and tissues when maintained at laboratory conditions with hormones, nutrients etc. under human control.

Tissue culture is the process that exploits the ability of plant cells to dedifferentiate and then redifferentiate to form a whole plant. Plant tissue culture is a key aspect of modern biotechnology, offering potential for progress and innovation in agriculture as well in forestry, industrial production of variety of plant secondary metabolites. The content of this course introduces variety of concepts about plant tissue culture, followed by laboratory setup and techniques, plant tissue culture media, concept and techniques of micropropagation, applied aspects of plant tissue culture techniques like callus culture, cell suspension culture, protoplast fusion, haploid production of plants also.

The germplasm conservation is a key process in further management of plant cells by cryopreservation. We authors have taken serious efforts to present the course matter in most simple way so that our students and our teachers will appreciate the style of presentation. The course matter contains variety of concepts, so we have tried our best to express this with our sincere efforts. We authors are thankful to our publisher Hon. Shri. Rangrao Patil, who has taken meticulous efforts to publish series of all titles.

We are also thankful to all the staff of Prashant Publications, Jalgoan for doing sincere efforts to present the alphabetical in a presentable form called Tissue Culture Technology. We all authors are thankful to several Publications, authors, organisations from where we have taken theoretical matter for our presentations. We are also thankful to our students and teacher friends which are our source of support and inspiration. We always take criticisms, suggestions and comments for further improvement of our books in future.