We are delighted to present this valuable book on “Bioinstrumentation” toF.Y.B.Sc. Biotechnology students with enormous excitement. This book was put together using Savitribai Phule Pune University’s New Revised Syllabus in keeping with the National Education Policy-2020 (NEP-2020) as of June 2024. Students in the F.Y.B.SC. Biotechnology course (Semester I-2P, under Skill Enhancement Course (SEC)).The purpose of this practical book is to offer teachers the framework they need to organize hands-on activities, effectively involve students in the teaching and learning process, and foster student’s various instrument handling skills. We believe that the book will assist student’s learn different laboratory techniques- Chromatography, Autoclave etc. and gain a brief understanding of the numerous devices -Spectrophotometer, Microscope, etc. and their uses in the laboratory. The most notable aspect of this book is that it offers a thorough strategy in an easy-to-memorize format.The Prashant Publications, Jalgaon, has shown a great deal of interest in publishing this book promptly and in an appealing format and for that we authors feel very grateful. We are thankful for the reader’s precise constructive criticism and suggestions on how to make this book better.