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This practical manual furnishes its level best, for the practical information required for completion of practical syllabus of S.Y.B.Sc. Botany Kavayitri Bahinabai ChaudhariNorth Maharashtra University, Jalgaon. We are confident that...

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Practical Botany (Based on BO-214 and BO-215)
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This practical manual furnishes its level best, for the practical information required for completion of practical syllabus of S.Y.B.Sc. Botany Kavayitri Bahinabai ChaudhariNorth Maharashtra University, Jalgaon. We are confident that this book will prove feasible assistance to the students and teachers while working in the laboratory. Adequate care has been taken regarding the correctness of the diagrams and matter written in a manual to eliminate the routine error as far as possible. This book is fairly illustrated and many possible details have been incorporated. The healthy suggestions and criticism from all corners of university jurisdictionare invited for subsequent edition.

Practical – 1 and 2............................................................7
Preparation of Digital Herbarium of some ethnobotanical plants (any five) and its techniques.
Practical – 3, 4 and 5.............................................16
Ethnobotany of some plants w. r. t. Botanical name, taxonomic description and distribution, phytochemistry and uses of:
i) Justicia adhathoda (Adulsa)
ii) Aegle marmelos (Bel)
iii) Azadirecta indica (Kadunimb)
iv) Butea monosperma (Palas)
v) Datura metal (Dhotara)
vi) Madhuka longifolia var. longifolia (Mahuva).
Practical – 6 and 7.................................................................29
Study of following plants with reference to their vernacular names, botanical names, family, plant parts used, uses, mode of preparation and administration from the local area with the help of plants or their parts or specimens.
A) Food plants: (Tubers): Dioscorea bulbifera (Kadukand)
B) Beverages: (Flowers): Madhuka longifolia (Mahu)
C) Oil Yielding: (Seed): Ricinus communis (Erand)
D) Fiber Yielding: (Stem): Helicterisisora (Murudsheng)
E) Bidi Wrapper: (Leaves): Diospyros melanoxylan (Tendu)
F) Tooth Brush: (Stem): Pongamia pinnata (Karanj), Cassia auriculata
(Avali), Vachellia nilotica (Babul), Azadirecta indica (Neem).
Practical – 8........................................................................40
Methods of Sterilization
Practical – 9 and 10...............................................................44
Nutritional media and their preparation
i) Bacterial- YEMA (Yeast Extract Mannitol Agar)
ii) Algal- Chu’s medium
iii) Fungal- PDA (Potato Dextrose Agar)
Practical – 11.....................................................................49
Methods of isolation and purification of microbial cultures.
Practical – 12.......................................................................51
Isolation of Rhizobium from leguminous root nodule. Practical – 13............................................................................54
Isolation of BGA from Paddy fields (Anabaena/ Nostoc/ Oscillatoria etc.).
Practical – 14........................................................................59
Mass cultivation of BGA.
Practical – 15.........................................................................61
To visit botanical garden/herbal garden/medicinal plant repositories.

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