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ZO 363 | Molecular Biology

T.Y.B.Sc. | Sem VI | Zoology

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T.Y.B.Sc. | Sem VI | ZO 363
Molecular Biology

It’s a great pleasure for us to present this book Molecular Biology (Zo-363, Semester VI) for T.Y.B.Sc. Zoology Students. This book is written as per the revised syllabus of Savitribai Phule Pune university, Pune to be implemented from June 2021.
We have tried to explain the concepts & information in lucid language so that students could understand it easily. Many senior faculty members have inspired us to perform this duty. We feel it’s not a new creations but presentation in simple form for the better understanding of students.

1. Nucleic Acids and Chromatin 
1.1 Structure of RNA & DNA
1.2 Types of RNA.
1.3 DNA as genetic material – evidences (Griffith’s, Avery et al., Hershey and Chase experiment), RNA as genetic material – TMV 4.
1.4 Structure of Chromatin, packaging of DNA, Heterochromatin, Euchromatin

2. Central Dogma of Molecular Biology 
2.1 DNA Replication – Semiconservative (Messelson and Stahl experiment), Basic mechanism of replication in prokaryotes and eukaryotes.
2.2 Transcription – 2.2.1. Basic mechanism of transcription in prokaryotes and eukaryotes, RNA polymerase enzyme in prokaryotes. 2.2.2. RNA modifications and processing (splicing – mRNA, modifications at 3’ and 5’ end).
2.3 Translation – Genetic code, properties of genetic code, Basic mechanism of Translation in E. coli and eukaryotic cells.

3. Lac Operon 
3.1 Lac Operon

4. DNA Repair Mechanism 
4.1 Photo repair, dark repair, base excision repair

5. Recombinant DNA Technology 
5.1 Introduction
5.2 Basic steps of Recombinant DNA Technology
5.3 Biological tools for recombinant DNA Technology
5.4 Restriction Enzymes
5.5 Cloning Vector
5.6 Polymerase Chain Reaction
5.7 DNA finger printing


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