BO-233 & 243 | Practical Botany
S.Y.B.Sc. | Sem III & IV | Botany
Dr A B Kadam, Dr B A Patil, Dr D D Ahire, Dr D M Mahajan, Dr M T Patil, Dr S K Aher, Dr S M Kamble, Prof B M GaykarRs.175.00
S.Y.B.Sc. | Sem III & IV | BO-233 & 243
BOTANY
Practical Botany
It is our great pleasure to introduce our book on Practical Botany for S.Y.B.Sc. as per CBCS pattern implemented from the year 2020. From this year onward the practical examination will be conducted in Sem III and Sem IV. To collect literature and protocols in systematic manner and it was first task with us and some how we have succeeded in that way.
To do practical means to understand by heart some small or complicated protocol and this is possible by having full involvement and enjoying the given task. If you understand the beauty of honest work then right mood is created in practical work. This helps to develop confidence in the personality that culminates a good success in the examination. We all expect that all students will read first introductory chapter, will act as guidelines to get correct direction to utilize very valuable time of practical class and your valued life too:
This book has two parts – First part includes protoc
Botany Practicals : An Introduction
Taxonomy of Angiosperms and Plant Ecology
Practical No. 1.
Study of tools of taxonomy and ecological instruments (any four each)
Practical No. 2.
Description of flowering plant in botanical terms
Practical No. 3.
Study of plant families (any four)
Practical No. 4.
Study of ecological adaptations in Hydrophytes with any two examples
Practical No. 5.
Study of ecological adaptations in Xerophytes with any two examples
Practical No. 6.
Study of vegetation by list count quadrat method
Plant Physiology
Practical No. 7.
Perform phytochemical test for starch and protein in germinating and non germinating seeds
Practical No. 8.
Isolation of Leaf Protein Concentration (LPC) from suitable plant material.
Practical No. 9.
Determination of Diffusion Pressure Deficit (DPD)
Practical No. 10.
Determine rate of transpiration under different conditions of Sunlight, Shade and Wind
Practical No. 11.
Demonstration of the following
a. Commercial biofertilizers, b. Imbibition in seeds, c. Ringing experiment, d. Arc Auxanometer, e. Spectrophotometer, f. Nitrogen fixing bacteria / BGA (specimen/ slide)
Practical No. 12.
Calculate seed germination percentage and vigor index
Practical No. 13.
Botanical excursion tour and visit to Floriculture industry / Soil testing center / Seed testing center
Plant Anatomy and Embryology
Practical No. 1.
Study of epidermal tissue system – non-glandular and glandular trichomes, multilayered epidermis, typical stomata (Dicotyledonous and Monocotyledonous).
Practical No. 2.
Study of mechanical tissues and their distribution in root, stem and leaves.
Practical No. 3.
Study of normal secondary growth in dicot stem – Annona/Moringa
(Double stained temporary preparation).
Practical No. 4.
Study of anomalous secondary growth in Bignonia and Dracaena stem
(Double stained temporary preparation).
Practical No. 5.
Study of tetrasporangiate anther and types of ovules with the help of permanent slides
Practical No. 6.
Study of dicot and monocot embryo
Plant Biotechnology
Practical No. 7.
Instruments/equipments used in plant tissue culture laboratory: Principle and working of Autoclave, oven, laminar air flow cabinet, micropipette, culture bottles/tubes with cotton plug
Practical No. 8.
Preparation & sterilization of MS medium
Practical No. 9.
Surface sterilization and Inoculation of nodal sector, leaf, anther and maize embryo
Practical No. 10.
Laboratory cultivation of Spirulina
Practical No. 11.
Demonstration practical on transgenic crops viz; Bt-Cotton, Golden rice
Practical No. 12.
Demonstration of principle and working of agarose gel electrophoresis, centrifuge, spectrophotometer
Practical No. 13.
Visit to plant tissue culture laboratory
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