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FYBSc Semester I

CH-103 & 203 | Practical Chemistry

F.Y.B.Sc. | Sem I & II | Chemistry

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F.Y.B.Sc. | Sem I & II | CH-103 & 203
Practical Chemistry

It’s a great pleasure for us to present this book for F.Y.B.Sc. Chemistry students of the Savitribai Phule Pune university, Pune. This book is written as per the revised syllabus (CBCS Pattern) of Savitribai Phule Pune university, Pune to be implemented from June 2019.
We have tried to explain the concepts & information in lucid language so that students could understand it easily. Many senior faculty members have inspired us to perform this duty. We feel it’s not a new creations but presentation in simple form for the better understanding of students.

CH-103 : Chemistry Practical


Practical No. 1 
Toxicity of compounds used in chemistry laboratory.
Practical No. 2 
Safety symbol on labels of pack of chemicals and its meaning.
Practical No. 3 
MSDS Sheets and Its use for Handling Hazardous Chemicals
Practical No. 4 
Precautions in Handling Hazardous Substances in Laboratory.


a) Thermochemistry (Any Three)
Practical No. 1 
To determination of heat capacity of calorimeter for different volumes.
Practical No. 2 
To determination of enthalpy of neutralization of hydrochloric acid with sodium hydroxide.
Practical No. 3 
To determine the enthalpy of ionization of acetic acid.
Practical No. 4 
To determine the integral enthalpy of solution of salts (KNO3/NH4Cl).
Practical No. 5 
To determine of enthalpy of hydration of copper sulphate.
Practical No. 6 
To study of the solubility of benzoic acid in water and determination of ΔH.
b) Ionic equilibria (Any Two)
Practical No. 7 
1. To measure pH of solutions like aerated drinks, fruit juices, shampoos and soaps using pH-meter.
Practical No. 8 
1. Measurement of the pH of buffer solutions and comparison of the values with theoretical values.
2. Prepare the different buffer solutions of sodium acetate-acetic acid and determine its buffer capacity
2. Prepare the different buffer solutions of NH4Cl- NH4OH- and determine its buffer capacity.


Practical No. 1 
To determine type and detection of extra elements (N, S, Cl, Br, I) in any Three organic compounds
(Containing up to two extra elements)
Practical No. 2 
Separation of constituents of mixtures by paper chromatography: (Any Two).
(a) Identify and separate the components of a given mixture of two amino acids by paper chromatography and measure the Rf value. (Glycine, aspartic acid, glutamic acid, tyrosine or any two amino acids / pigments from plant extract / two organic compounds)
(b) Identify and separate the sugars in mixture by paper chromatography and measure the Rf value.

CH-203: Chemistry Practical


I) Synthesis of Commercially Imp. Inorganic Compounds (Any Two)
Practical No. 1 
Synthesis of potash alum from aluminium metal (scrap Aluminium metal).
Practical No. 2 
Synthesis of Mohr’s salt Ferrous ammonium sulphate hexahydrate (FAS).
Practical No. 3 
Preparation of Dark red inorganic pigment: (Cu2O)
Practical No. 4 
Synthesis of FeSO4•7H2O

II) Volumetric Analysis (Any Two)

Practical No. 5
Estimation of sodium carbonate and sodium hydrogen carbonate present in a mixture.
Practical No. 6 
Estimation of basicity of boric acid or oxalic acid or citric acid hence determination of their equivalent weight.
Practical No. 7 
Estimation of water of crystallization in Mohr’s salt by titrating with KMnO4.

III) Analysis Of Commercial Products (Any Two)

Practical No. 8 
To determine the amount of calcium from given calcium tablet by complexometric titrationn
Practical No. 9 
To determine acid neutralizing capacity of antacids like Gelusil tablet/Gelusil syrup.
Practical No. 10 
To determine the amount of copper from sample of brass alloy by iodometrically
IV) To draw polar plots of s and p orbitals
Practical No. 11 
To sketch the polar plots of S and P orbital’s


I] Organic Purification Techniques (Two experiments from each technique)

Practical No. 1 
Purification of organic compounds by- i) crystallization (from water and alcohol) ii) distillation, iii) Sublimation
Organic Preparations: Derivatives (Any Two)
Practical No. 1 
Bromination of Cinnamic acid using Sodium bromide and Sodium bromate.
(Green Chemistry Approach) OR
Practical No. 1 
Bromination of acetanilide using KBr and Ceric ammonium nitrate in aqueous medium
Practical No. 2 
Semicarbazone derivatives of aldehydes and ketones
Oxime Oxime or 2,4-Dinitrophenylhydrazone derivative of aldehyde/ketone
APENDIX-I: Polar Graph Paper


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